Abstracts of Journal of Japanese Society for Magnesium
Research
J.J.S.Mg R. Vol. 14, No. 1 (1995)
ISSN 0913-4867 CODEN: MAGUEO
This is the Home Page of J.J.Mg R., made by Atsutane Ohta and
Masako Ohtsuki and donated as a public service by Paul
Mason.
Information:
J.J.Mg R. is the only special journal for magnesium
researchers in Japan.
Below are abstracts of eight papers published in the latest
J.J.Mg R..
Titles of papers:
- Effect of Light Exercise on serum Magnesium
in Human Subjects (Short report)
- Utilization of magnesium and calcium in
molokhia
- Magnesium and zinc in serum, erythrocyte
and urine of patients with essential hypertension and patients
with diabetes mellitus
- Magnesium, potassium and hormones in urines
of patients with essential hypertension
- Fermentation of soybean by Aspergillus
Usami Increases Magnesium Availability in Rats
- Cytosolic free calcium and magnesium
concentrations in smooth muscle cells of thoracic aorta
isolated from Mg-deficient rats
- The relationships between magnesium intake
and intake of foods, and nutrients intake among middle aged
men
- The balances of magnesium, calcium and
phosphorus in Japanese young males: comparison between two
different levels of magnesium intakes
[1] P. 7 - 11
Effect of Light Exercise on serum Magnesium in Human
Subjects (Short report)
Masahide Imaki*, Tsukasa Hara**, Yukie Yoshida*, Miho
Ohguri*, Seiki Tanada** and Misao Yamasaki***
*Osaka Prefectural Junior College of Health Science, **Faculty of
Pharmaceutical Sciences, Kinki University and ***Tokyo Dietitian
Academy
We report the effect of mild exercise on serum magnesium
level. 36 healthy female subjects were examined. A bicycle
ergometer was used. Amount of exercise corresponding to 30 and
40% of the maximum oxygen uptake were imposed for 10, 20 and 30
minutes, respectively, 3 times a week for 6 weeks. Serum
magnesium levels decreased slightly but not significantly. There
was no significant difference between serum magnesium levels of
the inactive groups and more active groups. We speculated that
mild exercise affects serum magnesium.
[2] P. 14 - 20
Utilization of magnesium and calcium in
molokhia
Mieko Kimura1, Takahiro Minaoka1, Yoshinori Itokawa1 and
Noriko Takagi2
1Department of social Medicine, Graduate School of Medicine,
Kyoto University and 2Arsoa Ohsho Co., Ltd.
Molokhia (Corchorus olitorius) leaves used in Egypt for
popular diets are rich in contents of minerals.@Evaluations of
magnesium (Mg) and calcium (Ca) in molokhia powder (MP) for
nutrient were examined.@Twenty four male Wister rats, 3 weeks
old, were divided into 3 groups, and given 3 kinds of the
synthetic diets; 1) 0% MP (control), 2) 10% MP, 3) 20% MP
(minerals and vitamins concentrations of the diets were finally
same, especially Mg concentration was high in 20% MP group) for 9
weeks.@Mg and Ca concentrations in plasma and tissues were
determined by ICP-AES. The body weight gain in 20% MP group rats
was significantly lower than these in other groups.@Mg
concentration in tibia of the 20% MP group rats was high compared
with that of the control group rats.@Mg concentration in plasma
of the 20% MP group rats was higher than those of other groups
rats.@Ca concentration of plasma of the 20% MP group rats was
lower than that of the control group rats.@The body weight gain,
and Mg and Ca concentrations in plasma and tissues of the rats
fed the diet containing 10% MP were not significantly changed
compared with these in rats fed the normal diet (0% MP).@These
results suggest that Mg and Ca in molokhia leaves could be
utilized as same as MgCO3 and CaHPO4 or CaCO3 in rats.
[3] P.13 - 20
Magnesium and zinc in serum, erythrocyte and urine of patients
with essential hypertension and patients with diabetes
mellitus
Noboru Saito*, Yuuko Konishi**, Akiko Kuchiba*** and
Toshiko Kannagi****
*Department of Internal Medicine, Miyazaki Aiwa Hospital,
**Medical Research Laboratory, Kochi Medical University, Nankoku
City, Kochi 783, Japan, ***Department of cooking Science, Kyoto
Women University, Kyoto city, Kyoto 606, ****Department of
Cardiovascular Diseases, Kyoto Medical Prevention Center, Kyoto
City, Kyoto 604
Three groups were compared about serum, erythrocyte or urine
magnesium (Mg) and zinc (Zn) levels in this investigation,
consisting of outpatients with essential hypertension,
outpatients with diabetes mellitus and control healthy subjects
(the controls). In total patients of both sexes serum Mg was
lowest in diabetic outpatients (diabetics) and then lower in
hypertensive outpatients (hypertensives) among these 3 groups.
Even in either male or female patients, serum Mg lower in
diabetics as compared to the controls. Serum Zn was lower in
total hypertensives or diabetics as compared to the controls.
Erythrocyte Mg in total patients and females was lower in
diabetics compared to hypertensives, when erythrocyte Zn in total
patients was lower in diabetics as compared to the controls.
Erythrocyte Zn in total patients was lower in either diabetics or
hypertensives as compared to the controls. In females erythrocyte
Mg and Zn were lower in diabetics than in the controls. In
addition, serum ionized Mg (Mg++) was lower in either totals or
females with diabetes mellitus as compared to the controls or
hypertensives. There were significantly positive correlation
serum Mg and Zn in females (r=0.405), between serum Mg and
erythrocyte Mg in both males and females (r=0.373 and 0.534,
respectively) and between serum Zn and erythrocyte Zn in females
(r=0.396). On the other hand Mg levels of 24-hour urine were
lower in hypertensives as compared to the other groups. Diabetics
showed both higher and lower levels of 24-hour urine, resulting
in the almost same level of urine Mg on an average to that of the
controls. Zn levels of 24-hour urine did not differ among 3
groups, indicating no increment of urine in diabetics.
[4] P. 35 - 44
Magnesium, potassium and hormones in urines of patients
with essential hypertension
Noboru Saito* and Yuuko Konishi**
*Department of Internal Medicine, Miyazaki Aiwa Hospital,
**Medical research Laboratory, Kochi Medical University, Kochi
783, Japan
In order to clarify the relationships among urine magnesium
(Mg), potassium (K), catecholamines and aldosterone, 105 subjects
aged 63.7+-10.7 years (M+-SD) were recruited in this study,
consisting of 41 male patients with essential hypertension, 45
female patients with essential hypertension, 8 male healthy
subjects and 11 female healthy subjects. Thereafter, the
additional subjects were enrolled to confirm the relations
between urine Mg and catecholamines, resulting in 178 subjects
aged 62.1+-11.7 years who consisted of 69 male hypertensives, 64
female hypertensives, 21 male healthies and 24 female healthies.
The collection of 24-hours urine was carried out in these
subjects to measure Mg, K, catecholamine and aldosterone. There
was found a significantly positive correlation between urine Mg
and K concentrations. A positive correlation between urine Mg and
aldosterone concentrations was also significant in hypertensives
of both sexes, indicating an intimate relation of K to
aldosterone compared to Mg. Furthermore, significantly positive
correlations were found between urine K and norepinephrine,
epinephrine or dopamine concentrations in male hypertensives. By
increasing numbers of subjects the correlations between urine Mg
and norepinephrine concentrations in both male hypertensives and
male healthies became significant. On the other hand there were
revealed significant correlations among norepinephrine,
epinephrine and dopamine in hypertensives of both sexes, and also
a positive correlation between urine dopamine and aldosterone
concentrations, showing an intimate relation of dopamine to
aldosterone. It is concluded from these findings that urine Mg
and K relate to urine catecholamines and aldosterone.
[5] P. 45 - 54
Fermentation of soybean by Aspergillus Usami Increases
Magnesium Availability in Rats
Miho Hirabayashi, Tohru Mitui, Antimon Ilyas and Hideo
Yano
Department of Animal Science, Faculty of Agriculture, Kyoto
University, Kyoto 606-01, Japan
To improve magnesium absorption through the reduction of
phytic acid content, defatted soybean was fermented by
aspergillus usami. After fermentation, phytate content was only
trace and the phytase activity was detected in the fermented
soybean. Apparent magnesium absorption was significantly higher
in rats fed the fermented soybean diet than animals fed the
intact soybean diet. In addition, even if sodium phytate was
added to the fermented soybean diet, apparent magnesium
absorption was as much as in rats fed the fermented soybean diet.
Magnesium solubility in the lower small intestine was higher in
rats fed the fermented soybean diet with or without adding
phytate than that in rats fed the soybean diet. Magnesium
concentrations in the femur of both of the fermented soybean
groups were higher than that of the soybean group. These results
suggested that fermentation of defatted soybean increased
magnesium bioavailability. It is also possible that the fermented
soybean can be used as phytase source.
[6] P. 55 - 62
Cytosolic free calcium and magnesium concentrations in
smooth muscle cells of thoracic aorta isolated from Mg-deficient
rats
Junko KANDA, Atsushi MIYAMOTO and Akira
NISHINO
Department of Veterinary Pharmacology, Faculty of Agriculture,
Kagosima University
Cytosolic free calcium ([Ca2+]i) and magnesium ([Mg2+]i)
concentrations were estimated in the smooth muscle cells of
thoracic aorta isolated from Mg-deficient and control rats using
fura-2/AM and mag-fura-2/AM, respectively. Adult male Wister rats
were fed with a Mg-deficient diet (0.001% Mg) for 30 days with
control groups (0.07% Mg). The thoracic aortic strips without
endothelium were loaded with fura-2/AM or mag-fura-2/AM in the
presence of 0.5 or 1.0mM Mg2+, then [Ca2+]i or [Mg2+]i was
measured in the same Mg2+ condition. [Ca2+]I of the thoracic
aorta isolated from Mg-deficient rats (254.9±13.1nM) in
the presence of 1.0mM Mg2+, however [Mg2+]I was not significantly
different between two groups in both Mg2+ levels. These results
suggest that [Ca2+]i, but not [Mg2+]i, of@thoracic aorta might be
increased by hypomagnesemia during dietary Mg-deficiency.
[7] P. 63 - 74
The relationships between magnesium intake and intake of
foods, and nutrients intake among middle aged men
Katsushi YOSHITA1, Masaji TABATA1, Yuko MORIKAWA1, Muneko
NISHIJO1, Masami SENMA1, Katsuyuki MIURA1, Hideaki NAKAGAWA1,
Kouichi HAYASHI2 and Takashi OHNISHI3
1Department of Public Health, Kanazawa Medical University,
2Health Promotion Division, Health and Welfare Department,
Ishikawa Prefectural Government and 3Komatsu Health center,
Ishikawa Prefectural Government
We performed a nutritional survey in 801 middle-aged men and
investigated total magnesium intake, magnesium density
(mg/1000kcal), magnesium intake by food groups and the
contribution ratio of food groups to total magnesium intake. The
mean daily intake of magnesium was 237mg. Subjects with higher
intake of magnesium or magnesium density took more magnesium from
pulses, fishes and shellfishes to total magnesium intake were
higher and that of cereals was lower in subjects with higher
intake of magnesium. Subjects with higher intake of magnesium per
energy intake showed higher intake of calcium, phosphorus, iron,
potassium and copper and lower intake of total energy and lipid
than those with lower intake.
[8] P. 75 - 82
The balances of magnesium, calcium and phosphorus in
Japanese young males: comparison between two different levels of
magnesium intakes
Hidemaro Takeyama, Noriko Kodama, Naomi Sato, Hiroyuki
Higuchi, Tokio Fuchi, Yutaka Yoshitake and Mamoru
Nishimuta
National Institute of Health and Nutrition, Shinjuku, Tokyo
(162)
The balance of magnesium (Mg), calcium (Ca), phosphorus (P)
were studied in 10 healthy males (aged from 19 to 26 yrs) under a
relatively low Mg diet with or without Mg supplement.
Experimental period was 15-days composed of a balance period of 8
days (two consecutive 4-days periods), with a 4-days pre-balance
and 3-days post-balance period. Throughout the experiment
subjects ingested the diets contained 150mg/d of Mg as
composition of common foods, five of them were additionally
supplemented with 300mg/d Mg oxide (180mg/d as Mg). Daily Mg
balances at dietary Mg intake of 150mg/d and 330mg/d were
4+-10mg/d and 11+-10mg/d respectively. Daily balance of Ca and P
were not affected by dietary Mg levels. This study indicated that
Mg balanced at wide range of dietary Mg levels.
Address of Journal of Japanese Society for Magnesium
Research
Prof. Yoshinori Itokawa
Japanese Society for Magnesium Research
Kinki local invention center (a nonprofit corporation)
Society planning office
14 Yoshidakawara-chyo Sakyou-ku Kyouto Japan 606
Phone & Fax 81(75)751-7599
Information of The 16th Annual meeting of the Japanese
Society for Magnesium Research
Date: November 15 - 16, 1996
Place: The National Institute of Health and Nutrition
Nov. 15 Public Symposium (Magnesium and Health)
Nov. 16 General announcement
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